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Experimental Research

Quantification of tumor virulence based upon competition between dissimilar subcultured cancer cell lines

Author:

Philip Weintraub

Department of Medicine, NYU Langone Health, 791 Park Avenue, New York, NY 10021, US
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Abstract

Purpose: A direct quantitative metric that represents the intrinsic virulent properties of a particular tumor is absent, and the development of such a numeric modifier would balance the disproportionate weight of qualitative elements that presently monopolize the nomenclature of cancer. To address this shortcoming, an in vitro model was developed to study the competitive nature of dissimilar cancer cell lines.

Methods: A prospective series of surgically excised and pathologically confirmed malignant tumors were separated from their adherent state and subcultured according to international standards. After a total of 7 different tumor cell lines were established, each cell line was paired with a dissimilar cancer cell line, and by using all possible combinations, a total of 21 unique tumor pairs were assessed over 7 days after plating on diametrically opposed sections of gridded Petri dishes containing enriched growth medium. Each dish was then examined for changes in tumor cell position on the grid, and a scoring system was established that awarded points for aggressive behaviors.

Results: Ovarian cancer, lymphoma, and lung cancers demonstrated overall dominance, with wins in at least 2/3 of their matches. Breast and kidney cancers were intermediate in performance, and gastric and colon cancers were subordinate in the majority of their matches.

Discussion: This pilot experiment revealed that intercellular competition exists between previously untreated, subcultured, and dissimilar tumor cells in this in vitro assay. Moreover, such intercellular competition and the unilateral dominance of specific cancer tissue types have not been previously reported in the literature.

How to Cite: Weintraub P. Quantification of tumor virulence based upon competition between dissimilar subcultured cancer cell lines. International Journal of Surgery: Oncology. 2021;5(6):e96. DOI: http://doi.org/10.1097/IJ9.0000000000000096
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Published on 19 Jan 2021.
Peer Reviewed

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